Phloroglucinol-assisted low-level laser therapy (LLLT) to prevent recurrence of urethral stricture

Yeachan Lee; Hyejin Kim; Hyun Wook Kang

doi:10.1117/12.2507114

26 February 2019 Phloroglucinol-assisted low-level laser therapy (LLLT) to prevent recurrence of urethral stricture

Yeachan Lee, Hyejin Kim, Hyun Wook Kang

Proceedings Volume 10852, Therapeutics and Diagnostics in Urology 2019; 1085208 (2019) https://doi.org/10.1117/12.2507114
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Urethral stricture, also called urethral stenosis, is an abnormal narrowing of the urethra due to scarring on the urinary wall caused by inflammation or trauma. Although various surgical treatments have been developed such as urethrotomy and urethroplasty, the recurrence rate is almost 40 percent due to development of fibrosis in the urethra. The purpose of the current study was to evaluate the effect of phloroglucinol-assisted low-level laser therapy (LLLT) on inhibition of fibrosis in in vitro. LLLT is one of the promising methods for fibrosis treatment, and phloroglucinol has an anti-fibrotic effect. NIH/3T3 fibroblast cells were cultured in a medium containing transforming growth factor (TGF)-β1 to induce differentiation of the fibroblasts. Laser (635 nm at 375 mJ/cm²) and phloroglucinol (100 μg/ml) were treated separately or simultaneously after TGF-β1 was treated. MTT and BrdU assay presented the inhibitory effect of the combined treatment on cell proliferation. An inhibitory effect on migration speed was confirmed by wound healing assay. Confocal immunofluorescence images showed that the combined treatment inhibited the expression of α-smooth muscle action (sma) and type-1 collagen. Moreover, Western blot analysis demonstrated that α-sma, collagen-1, and TGF-β1 as the key factors of fibrosis were suppressed by the combined treatment. Therefore, phloroglucinol-assisted LLLT may be an effective treatment to prevent the recurrence of urethral stricture. In vivo tests will be conducted in canine models for pre-clinical evaluations.

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Yeachan Lee, Hyejin Kim, and Hyun Wook Kang "Phloroglucinol-assisted low-level laser therapy (LLLT) to prevent recurrence of urethral stricture", Proc. SPIE 10852, Therapeutics and Diagnostics in Urology 2019, 1085208 (26 February 2019); https://doi.org/10.1117/12.2507114

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KEYWORDS

Laser therapeutics

Collagen

Confocal microscopy

Absorbance

Proteins

In vivo imaging

Wound healing

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