Antimicrobial photodynamic therapy applied to inactivation of salmonella enterica and staphylococcus aureus

Ranniele L. V. Silva; Ialy A. Moura; Juliana O. Moraes; Cintia T. Andrade

doi:10.1117/12.2508072

1 March 2019 Antimicrobial photodynamic therapy applied to inactivation of salmonella enterica and staphylococcus aureus

Ranniele L. V. Silva, Ialy A. Moura, Juliana O. Moraes, Cintia T. Andrade

Author Affiliations +

Proceedings Volume 10876, Optical Interactions with Tissue and Cells XXX; 108761G (2019) https://doi.org/10.1117/12.2508072
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Salmonella enterica is a gram-negative pathogen with great relevance in the food industry, related to food gastroenteritis. Staphylococcus aureus is a gram-positive bacterium that causes intestinal infection, vomiting, and diarrhea when its toxins are ingested. One way to combat these pathogens is photodynamic inactivation (PDI), which consists of the interaction of three elements: photosensitizer, light and molecular oxygen. This interaction promotes the formation of reactive oxygen species, which leads to cell death through necrosis and apoptosis. The present study was divided into four groups: control, light, photosensitizer, and PDI, in order to evaluate the effect of the individually applied and combined elements. In addition, a comparison between the responses of different types of bacteria was performed. The photosensitizer used was protoporphyrin IX, produced through its precursor aminolevulinic acid (ALA). The ALA was applied at concentrations of 0.4 and 0.7 μM, and incubation time from one to four hours. Irradiation was performed at 630 nm and a fluence of 12, 36 and 72 J/cm² . The results indicated that the photosensitizer concentrations were inefficient to cause total cell death, however, gram-positive bacteria were more susceptible to the technique. Photodynamic antimicrobial therapy is an alternative to existing techniques, presenting a great cost-benefit and having the advantage of being ecologically correct since it only involves non-toxic elements to cause the total destruction of the bacteria.

Citation Download Citation

Ranniele L. V. Silva, Ialy A. Moura, Juliana O. Moraes, and Cintia T. Andrade "Antimicrobial photodynamic therapy applied to inactivation of salmonella enterica and staphylococcus aureus", Proc. SPIE 10876, Optical Interactions with Tissue and Cells XXX, 108761G (1 March 2019); https://doi.org/10.1117/12.2508072

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available