Raman imaging of α-synuclein aggregates in a rat model of Parkinson’s disease

Eva Brauchle; Fide Sevgi; Daniel Carvajal Berrio; Katja Schenke-Layland; Nicolas Casadei; Olaf Riess; Madhuri S. Salker; Yogesh Singh

doi:10.1117/12.2509751

4 March 2019 Raman imaging of α-synuclein aggregates in a rat model of Parkinson’s disease

Eva Brauchle, Fide Sevgi, Daniel Carvajal Berrio, Katja Schenke-Layland, Nicolas Casadei, Olaf Riess, Madhuri S. Salker, Yogesh Singh

Author Affiliations +

Proceedings Volume 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII; 1088116 (2019) https://doi.org/10.1117/12.2509751
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Aggregates of misfolded α-Synuclein in the brain represent a hallmark of Parkinson’s disease (PD). In patients and animal models, phosphorylated α-Synuclein was detected in the gut, hence, raising the hypothesis that early-stage PD could be diagnosed based on colon tissues. Marker-independent technologies represent an ideal method to monitor disease progression and potentially detect early-stage aggregated α-Synuclein in vivo. Here, formalin-fixed, paraffinembedded colon tissues of a transgenic rat model were analyzed using Raman imaging. Detailed spectral and imagebased analysis was performed indicating the major spectral shifts that alter in PD rat tissues in the amide I region. Peak fitting and multivariate analysis specified an increase of β-sheet proteins in transgenic rat colon compared with wild-type colon. In summary, Raman imaging is capable to detect α-Synuclein aggregates in colon tissues of a PD rat model, indicating that it could be a useful tool to support diagnosis in PD pathology.

Conference Presentation

Citation Download Citation

Eva Brauchle, Fide Sevgi, Daniel Carvajal Berrio, Katja Schenke-Layland, Nicolas Casadei, Olaf Riess, Madhuri S. Salker, and Yogesh Singh "Raman imaging of α-synuclein aggregates in a rat model of Parkinson’s disease", Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 1088116 (4 March 2019); https://doi.org/10.1117/12.2509751

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