Development of microscopic adaptive optics using image correlation

Yusuke Honma; Noriaki Miura; Hayao Kikuchi; Masayuki Hattori; Yosuke Tamada; Atsushi Matsuda

doi:10.1117/12.2509165

20 February 2019 Development of microscopic adaptive optics using image correlation

Yusuke Honma, Noriaki Miura, Hayao Kikuchi, Masayuki Hattori, Yosuke Tamada, Atsushi Matsuda

Proceedings Volume 10886, Adaptive Optics and Wavefront Control for Biological Systems V; 1088617 (2019) https://doi.org/10.1117/12.2509165
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Adaptive optics (AO) is a promising technique for correcting wavefront errors induced by complex structures of biological samples which significantly causes image degradation. We develop a microscopic AO system with a Shack-Hartmann wavefront sensor based on image correlation. The correlation-based wavefront sensing is feasible using an extended object under bright-field illumination, as well as spot fluorescence. To make the correlation-based sensing more reliable, we newly introduce a technique of excluding sub-images with insufficient quality. We show experimental results under a variety of conditions for objects, light sources, and wavefront error sources. In any cases, we confirmed that the AO system effectively worked so as to improve image qualities.

Citation Download Citation

Yusuke Honma, Noriaki Miura, Hayao Kikuchi, Masayuki Hattori, Yosuke Tamada, and Atsushi Matsuda "Development of microscopic adaptive optics using image correlation", Proc. SPIE 10886, Adaptive Optics and Wavefront Control for Biological Systems V, 1088617 (20 February 2019); https://doi.org/10.1117/12.2509165

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