Polarization wavefront shaping for quantitative phase contrast imaging by axially-offset differential interference contrast (ADIC) microscopy

Changqin Ding; Chen Li; Fengyuan Deng; Garth J. Simpson

doi:10.1117/12.2509929

4 March 2019 Polarization wavefront shaping for quantitative phase contrast imaging by axially-offset differential interference contrast (ADIC) microscopy

Changqin Ding, Chen Li, Fengyuan Deng, Garth J. Simpson

Author Affiliations +

Proceedings Volume 10887, Quantitative Phase Imaging V; 1088728 (2019) https://doi.org/10.1117/12.2509929
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Axially-offset differential interference contrast (ADIC) microscopy was developed for quantitative phase contrast imaging (QPI) by using polarization wavefront shaping approach with a matched pair of micro-retarder arrays. In ADIC microscopy, wavefront shaping with a micro-retarder array (μRA) produces a pattern of half-wave retardance varying spatially in the azimuthal orientation of the fast-axis. For a linearly polarized input beam, the polarization pattern induced from the linearly polarized plane wave through the μRA is identical to the interference between a slightly diverging right circularly polarized (RCP) and a slightly converging left circularly polarized (LCP) plane wave. Using a 10× objective, two axially offset foci separated by 70 μm are consequently generated from the patterned wavefront with orthogonal polarization states, serving as the sample and reference focal planes respectively for QPI. A paired μRA in transmission coherently recombines the two orthogonal components to recover the incident polarization state in the absence of sample. The large spatial offset (roughly 1/10 of the field of view) between the two foci provides a stable and uniform reference. Quantitative phase contrast images are directly recovered from sample-scan measurements with a single-channel detector and lock-in amplification with fast polarization modulation. This method has been successfully used for bio-sample imaging, nanoparticle detection and refractive index calculation of silica microbeads.

Citation Download Citation

Changqin Ding, Chen Li, Fengyuan Deng, and Garth J. Simpson "Polarization wavefront shaping for quantitative phase contrast imaging by axially-offset differential interference contrast (ADIC) microscopy", Proc. SPIE 10887, Quantitative Phase Imaging V, 1088728 (4 March 2019); https://doi.org/10.1117/12.2509929

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