Resolution optimized prism-based SPR imaging for the study of individual bacteria interaction with surface

Marine Boulade; Thierry Livache; Paul G. Charette; Michael Canva; Loïc Leroy

doi:10.1117/12.2507770

7 March 2019 Resolution optimized prism-based SPR imaging for the study of individual bacteria interaction with surface

Marine Boulade, Thierry Livache, Paul G. Charette, Michael Canva, Loïc Leroy

Author Affiliations +

Proceedings Volume 10894, Plasmonics in Biology and Medicine XVI; 108940J (2019) https://doi.org/10.1117/12.2507770
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Bacterial pathogen contamination is the leading cause of both foodborne and hospital-acquired diseases. Therefore, there is a constant need for more effective, reliable and easy-to-use microbiology study techniques and detection systems. This is critical, as pathogenic contamination has become a central issue in the food industry and healthcare. This paper describes the novel use of resolution-optimized prism-based surface plasmon resonance imaging (resolution around the size of a bacteria) and data processing to further understand the behavior of individual bacteria near specifically engineered surfaces. We show that our technique is effective for both the dynamic study of individual bacteria behavior near interface on a statistically representative sample, and their interactions with chemically functionalized surfaces.

Conference Presentation

Citation Download Citation

Marine Boulade, Thierry Livache, Paul G. Charette, Michael Canva, and Loïc Leroy "Resolution optimized prism-based SPR imaging for the study of individual bacteria interaction with surface", Proc. SPIE 10894, Plasmonics in Biology and Medicine XVI, 108940J (7 March 2019); https://doi.org/10.1117/12.2507770

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