Cell imaging with squaraine dye based on two-photon excitation fluorescence imaging

Junxian Geng; Rongxing Yi; Junle Qu; Liwei Liu

doi:10.1117/12.2506530

15 November 2018 Cell imaging with squaraine dye based on two-photon excitation fluorescence imaging

Junxian Geng, Rongxing Yi, Junle Qu, Liwei Liu

Proceedings Volume 10964, Tenth International Conference on Information Optics and Photonics; 109646G (2018) https://doi.org/10.1117/12.2506530
Event: Tenth International Conference on Information Optics and Photonics (CIOP 2018), 2018, Beijing, China

Abstract

Two-photon excitation fluorescence imaging (TPEFI) is widely used in biomedicine because it has advantages of low cytotoxicity, high resolution and deep imaging depth. In this experiment, OVCAR-3 ovarian cancer cells were labelled with the squaraine dye and detected by confocal laser scanning microscope ( Leica TCS SP8 )for single-photon and two-photon imaging. The results showed that the squaraine dye can be used well to two-photon imaging.

Citation Download Citation

Junxian Geng, Rongxing Yi, Junle Qu, and Liwei Liu "Cell imaging with squaraine dye based on two-photon excitation fluorescence imaging", Proc. SPIE 10964, Tenth International Conference on Information Optics and Photonics, 109646G (15 November 2018); https://doi.org/10.1117/12.2506530

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