Developing a novel hyperspectral imaging cryomacrotome for whole body fluorescence imaging

Boyu Meng; Brook K. Byrd; Dennis J. Wirth; Rendall R. Strawbridge; Scott C. Davis

doi:10.1117/12.2544497

21 February 2020 Developing a novel hyperspectral imaging cryomacrotome for whole body fluorescence imaging

Boyu Meng, Brook K. Byrd, Dennis J. Wirth, Rendall R. Strawbridge, Scott C. Davis

Proceedings Volume 11219, Visualizing and Quantifying Drug Distribution in Tissue IV; 112190M (2020) https://doi.org/10.1117/12.2544497
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The ability to directly measure whole-body fluorescence can enable tracking of labeled cells, metastatic spread, and drug bio-distribution. We describe the development of a new hyperspectral imaging whole body cryomacrotome designed to acquire 3-D fluorescence volumes in large specimens (whole animals) at high resolution. The use of hyperspectral acquisition provides full spectra at every voxel, enabling spectral decoupling of multiple fluorohpores and autofluorescence. We present examples of tissue spectra and spectral fitting in a rodent glioma xenograft.

Conference Presentation

Citation Download Citation

Boyu Meng, Brook K. Byrd, Dennis J. Wirth, Rendall R. Strawbridge, and Scott C. Davis "Developing a novel hyperspectral imaging cryomacrotome for whole body fluorescence imaging", Proc. SPIE 11219, Visualizing and Quantifying Drug Distribution in Tissue IV, 112190M (21 February 2020); https://doi.org/10.1117/12.2544497

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