Long-term Brillouin imaging of live cells with reduced photodamage at 660nm wavelength reveals cell mechanical response to environmental cues (Conference Presentation)

Milos Nikolic; Kandice Tanner; Giuliano Scarcelli

doi:10.1117/12.2547661

9 March 2020 Long-term Brillouin imaging of live cells with reduced photodamage at 660nm wavelength reveals cell mechanical response to environmental cues (Conference Presentation)

Milos Nikolic, Kandice Tanner, Giuliano Scarcelli

Author Affiliations +

Proceedings Volume 11242, Optical Elastography and Tissue Biomechanics VII; 112420K (2020) https://doi.org/10.1117/12.2547661
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

In Brillouin microscopy, absorption-induced photodamage of incident light is the primary limitation on signal-to-noise ratio in many practical scenarios. 660 nm may represent an optimal wavelength for Brillouin microscopy as it offers minimal absorption-mediated photodamage at high Brillouin scattering efficiency. We demonstrate that live cells are ~80 times less susceptible to the 660 nm incident light compared to 532 nm light, which overall allows Brillouin imaging with more than 30 times higher signal intensity. We apply this improved Brillouin microscope to analyze the response of human glioblastoma cells to a range of in vitro biomimetic environments.

Conference Presentation

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Milos Nikolic, Kandice Tanner, and Giuliano Scarcelli "Long-term Brillouin imaging of live cells with reduced photodamage at 660nm wavelength reveals cell mechanical response to environmental cues (Conference Presentation)", Proc. SPIE 11242, Optical Elastography and Tissue Biomechanics VII, 112420K (9 March 2020); https://doi.org/10.1117/12.2547661

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KEYWORDS

Live cell imaging

Microscopy

Laser scattering

Light scattering

Environmental sensing

Microscopes

Scattering

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