3,3’-thiodipropanol as a refractive index matching mounting medium for fluorescence microscopy

Milvia Iris Alata Tejedo; Juan Carlos Martinez Cervantes; Valeria Piazza

doi:10.1117/12.2551802

17 February 2020 3,3’-thiodipropanol as a refractive index matching mounting medium for fluorescence microscopy

Milvia Iris Alata Tejedo, Juan Carlos Martinez Cervantes, Valeria Piazza

Author Affiliations +

Proceedings Volume 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII; 1124514 (2020) https://doi.org/10.1117/12.2551802
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

In this work, several tests of the performance of 3,3’-thiodipropanol (TDP) as a mounting medium for fluorescence microscopy of biological samples were performed. Besides optical properties like the dispersion curve of TDP and the effect of the embedding medium on the fluorescence of commonly used dyes, the interaction with biological specimens, including the labeling of filamentous actin with fluorescent phalloidins, was tested: TDP showed to represent an interesting alternative to commercial mounting media. By mixing TDP with 2,2’-thiodiethanol (TDE), it was possible not only to fine tune the refractive index of the resulting solution, but to preserve the compatibility with fluorescent phalloidins.

Conference Presentation

Citation Download Citation

Milvia Iris Alata Tejedo, Juan Carlos Martinez Cervantes, and Valeria Piazza "3,3’-thiodipropanol as a refractive index matching mounting medium for fluorescence microscopy", Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 1124514 (17 February 2020); https://doi.org/10.1117/12.2551802

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