Volumetric imaging of synaptic activity at depth by adaptive optical Bessel focus scanning multiphoton microscopy (Conference Presentation)

Wei Chen; Ryan Natan; Yuhan Yang; Na Ji

doi:10.1117/12.2551572

11 March 2020 Volumetric imaging of synaptic activity at depth by adaptive optical Bessel focus scanning multiphoton microscopy (Conference Presentation)

Wei Chen, Ryan Natan, Yuhan Yang, Na Ji

Author Affiliations +

Proceedings Volume 11248, Adaptive Optics and Wavefront Control for Biological Systems VI; 1124803 (2020) https://doi.org/10.1117/12.2551572
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

An enabling technology for the monitoring of neural activity, multiphoton microscopy with Bessel focus scanning is a high-speed volumetric imaging method with subcellular lateral resolution. Similar to many other optical microscopy techniques, however, its axially extended Bessel-like focus experiences sample-induced optical aberrations, which lead to reduced resolution and image contrast at depth. In this study, we demonstrated an adaptive optical Bessel focus scanning multiphoton microscope with pupil-segmentation-based wavefront sensing and highly efficient sample-plane aberration correction. Applying it to mouse brain imaging in vivo, we observed up to threefold signal enhancement for functional spine imaging at depth.

Conference Presentation

Citation Download Citation

Wei Chen, Ryan Natan, Yuhan Yang, and Na Ji "Volumetric imaging of synaptic activity at depth by adaptive optical Bessel focus scanning multiphoton microscopy (Conference Presentation)", Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 1124803 (11 March 2020); https://doi.org/10.1117/12.2551572

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