Label-free super-resolution microsphere-assisted microscopy of biological samples (Conference Presentation)

Sylvain Lecler; Stéphane Perrin; Giorgio Quaranta; Nadia Messaddeq; Nicolas Lemercier; Jean-Luc Vonesch; Paul Montgomery

doi:10.1117/12.2548540

10 March 2020 Label-free super-resolution microsphere-assisted microscopy of biological samples (Conference Presentation)

Sylvain Lecler, Stéphane Perrin, Giorgio Quaranta, Nadia Messaddeq, Nicolas Lemercier, Jean-Luc Vonesch, Paul Montgomery

Author Affiliations +

Proceedings Volume 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020; 1125112 (2020) https://doi.org/10.1117/12.2548540
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Most of the recent super-resolution imaging techniques developed for biology are based on fluorescence properties which generally require toxic dyes. We show how super-resolution can be obtained without dyes by simply adding 20-micrometer-diameter dielectric spheres on the sample under a microscope objective. The microsphere behaves as a non-classical lens, collecting evanescent waves in a full-field imaging process. Resolutions of up to between /5 and /7 can be reached in air and in immersion, respectively. For translucent biological samples, a dark-field optical setup is proposed. Performance of the label-free super-resolution technique is demonstrated through the imaging of fixed nerve sections from a mouse embryo brain.

Conference Presentation

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Sylvain Lecler, Stéphane Perrin, Giorgio Quaranta, Nadia Messaddeq, Nicolas Lemercier, Jean-Luc Vonesch, and Paul Montgomery "Label-free super-resolution microsphere-assisted microscopy of biological samples (Conference Presentation)", Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 1125112 (10 March 2020); https://doi.org/10.1117/12.2548540

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KEYWORDS

Super resolution

Optical spheres

Microscopy

Microscopes

Refractive index

Biology

Dielectrics

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