Presentation
5 March 2021 Characterization of atherosclerotic plaque components using multispectral fluorescence lifetime imaging microscopy
Author Affiliations +
Abstract
We developed a high-precision multispectral fluorescence lifetime imaging microscopy (FLIM) for label-free immune-histologic imaging of atherosclerotic plaques. With images of fluorescence lifetimes and intensity ratios between different channels, we could characterize various plaque components of coronary arteries that are related to immunohistochemistry results. Correlative FLIM-immunohistochemistry validation revealed significant associations between plaque components and multispectral FLIM parameters. The machine learning algorithm, trained with co-registered FLIM-immunohistochemistry datasets, allowed automated visualization of multiple atherosclerotic components from FLIM image of an unstained section. We anticipate that the multispectral FLIM can be widely used to assess biochemical components of various biological tissues, including atherosclerotic plaques.
Conference Presentation
© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jeongmoo Han, Sunwon Kim, Hyun Jung Kim, Hyeong Soo Nam, Jin Won Kim, and Hongki Yoo "Characterization of atherosclerotic plaque components using multispectral fluorescence lifetime imaging microscopy", Proc. SPIE 11636, Optical Biopsy XIX: Toward Real-Time Spectroscopic Imaging and Diagnosis, 116360B (5 March 2021); https://doi.org/10.1117/12.2578435
Advertisement
Advertisement
KEYWORDS
Fluorescence lifetime imaging

Microscopy

Multispectral imaging

Luminescence

Arteries

Collagen

Tissues

Back to Top