Metabolic FLIM at the dawn of clinical application

Wolfgang Becker; Lukas Braun

doi:10.1117/12.2578319

5 March 2021 Metabolic FLIM at the dawn of clinical application

Wolfgang Becker, Lukas Braun

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480J (2021) https://doi.org/10.1117/12.2578319
Event: SPIE BiOS, 2021, Online Only

Abstract

The fluorescence lifetime of NADH in live cells is an indicator of the metabolic state and, consequently, a discrimination parameter between normal and tumor cells. However, the fluorescence lifetime of NADH also depends on the cell type and on the mitochondrial pH. The task is therefore to find a FLIM signature that describes the metabolic state without being influenced by other parameters. We show that the ratio of the amplitudes of the decay components, a1/a2, of NADH is an absolute parameter for discriminating normal cells from tumor cells, and outline the technical requirements for metabolic FLIM for clinical use.

Conference Presentation

Citation Download Citation

Wolfgang Becker and Lukas Braun "Metabolic FLIM at the dawn of clinical application", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480J (5 March 2021); https://doi.org/10.1117/12.2578319

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