Multiphoton confocal imaging of mammalian cells in presence of Zinc Nitride nanoparticle

Prabha Soundharraj; Durgalakshmi Dhinasekaran; Ganesan Singaravelu; Anandh Sundaramoorthy; Aruna Prakasa Rao

doi:10.1117/12.2583362

5 March 2021 Multiphoton confocal imaging of mammalian cells in presence of Zinc Nitride nanoparticle

Prabha Soundharraj, Durgalakshmi Dhinasekaran, Ganesan Singaravelu, Anandh Sundaramoorthy, Aruna Prakasa Rao

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480O (2021) https://doi.org/10.1117/12.2583362
Event: SPIE BiOS, 2021, Online Only

Abstract

Fluorescence labelling is one of the significant properties for optical bioimaging and biosensing applications. The development in the field of nanotechnology has created enormous conveniences in biomedical research. The fluorescence enhancement of mammalian cell in presence of zinc nitride colloidal nanoparticle was imaged using multiphoton confocal microscope. The statistical significance in the presence and absence of nanoparticle evidenced the fluorescence intensity enhancement in the cells. In order to suppress the background signal and increase the penetration depth, the multiphoton technique is utilized by 740 nm excitation to examine the enhancement potential of Zn₃N₂ colloidal nanoparticles towards cell structures. Hence, we suggest that, the unique feature of inorganic nanomaterial is a promising material for probing cells for future diagnostic applications.

Citation Download Citation

Prabha Soundharraj, Durgalakshmi Dhinasekaran, Ganesan Singaravelu, Anandh Sundaramoorthy, and Aruna Prakasa Rao "Multiphoton confocal imaging of mammalian cells in presence of Zinc Nitride nanoparticle", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480O (5 March 2021); https://doi.org/10.1117/12.2583362

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