1Politecnico di Milano (Italy) 2CNR-Istituto di Fotonica e Nanotecnologie (Italy) 3Ctr. for Nano Science and Technology, Istituto Italiano di Tecnologia (Italy)
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
Multispectral Fluorescence Lifetime Imaging Microscopy (FLIM) is a fundamental tool to study multifold processes in biology and material science. The growing demand for acquisition time reduction requires the parallel acquisition of a multi-dimensional dataset and the exploitation of compressive sensing techniques. In this work we present a multispectral FLIM set-up based on wide-field structured illumination coupled with a spectrometer and a novel time-resolved parallel 18x1 SPAD array detector, working in a single pixel camera scheme. We show the system characterization and its imaging properties varying the compression ratio.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
A. Ghezzi, A. Farina, A. Bassi, G. Valentini, I. Labanca, G. Acconcia, I. Rech, C. D'Andrea, "Time-resolved multi-spectral wide-field fluorescence lifetime imaging microscopy with a SPAD array detector," Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164905 (5 March 2021); https://doi.org/10.1117/12.2578683