Spatial light modulator approaches for reducing scattered and out-of-focus light

Yao L Wang; Sabrina C Civale; Noa W. F. Grooms; Samuel H. Chung

doi:10.1117/12.2584545

5 March 2021 Spatial light modulator approaches for reducing scattered and out-of-focus light

Yao L Wang, Sabrina C Civale, Noa W. F. Grooms, Samuel H. Chung

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 116490S (2021) https://doi.org/10.1117/12.2584545
Event: SPIE BiOS, 2021, Online Only

Abstract

Stray light, including scattered and out-of-focus light, can obscure the imaging of dim structures. We added a spatial light modulator at the field stop of our widefield fluorescence microscope to spatially control the illumination and are pursuing several approaches to reduce stray light. We demonstrate confocal capability and a combined >50x signal-to-background ratio improvement by using existing and novel techniques in illumination modulation and image postprocessing. Our approaches offer a simple and low-cost method for adapting existing microscopes to greatly improve the visibility of dim structures that are obscured by bright neighbors.

Conference Presentation

Citation Download Citation

Yao L Wang, Sabrina C Civale, Noa W. F. Grooms, and Samuel H. Chung "Spatial light modulator approaches for reducing scattered and out-of-focus light", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 116490S (5 March 2021); https://doi.org/10.1117/12.2584545

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