Nanometric resolution intracellular dynamics study using quadriwave lateral shearing interferometry

Roman Zinchuk; Youenn Le Gal; Julien Savatier; Serge Monneret; Benoit Wattellier

doi:10.1117/12.2583089

5 March 2021 Nanometric resolution intracellular dynamics study using quadriwave lateral shearing interferometry

Roman Zinchuk, Youenn Le Gal, Julien Savatier, Serge Monneret, Benoit Wattellier

Proceedings Volume 11653, Quantitative Phase Imaging VII; 1165319 (2021) https://doi.org/10.1117/12.2583089
Event: SPIE BiOS, 2021, Online Only

Abstract

Interaction between intracellular compartments is based on complex vesicular transport processes. Fluorescence based techniques now reach single molecule resolution in living cells. To avoid fluorophore photobleaching limitations, quantitative phase imaging techniques are conventionally used to produce long-term high contrast cell images. We used an improved wave front sensing set-up called QWLSI-HD, which resolution is high enough to reach nanometric resolution for vesicle localization. We also added specificity to quantitative phase imaging by training artificial intelligence algorithms to recognize intracellular vesicles. We compared performances between the algorithms and also between fixed and living samples.

Conference Presentation

Citation Download Citation

Roman Zinchuk, Youenn Le Gal, Julien Savatier, Serge Monneret, and Benoit Wattellier "Nanometric resolution intracellular dynamics study using quadriwave lateral shearing interferometry", Proc. SPIE 11653, Quantitative Phase Imaging VII, 1165319 (5 March 2021); https://doi.org/10.1117/12.2583089

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