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We provide a multiple-sinusoid modulated optical tweezers based method to perform microrheology of a sample over a large frequency band in a one shot. So we feed the trapped particle with a square wave and a superposition of multiple-sinusoid of specific frequency and amplitude to achieve our purpose towards active microrheology. To maintain the SNR upto a certain level for the entire frequency range we increase the amplitude at high frequency in our setup. We measure the parameters of the complex fluid by extracting the phase responses at each frequency of the particle with a high SNR value compared to passive microrheology. We perform this method for a various concentrations of polyacrylamide-water solution and obtain a good agreement of the fluid parameters with the theoretical values.
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Avijit Kundu, Raunak Dey, Shuvojit Paul, Ayan Banerjee, "Microrheology over a broad frequency range probing multiple-sinusoid oscillating optical tweezer," Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVIII, 117980D (1 August 2021); https://doi.org/10.1117/12.2596296