Label-free imaging of live migrasome formation using optical diffraction tomography

Yanqing Xu; Yanping He; Shiyuan Wei; Liting Duan; Renjie Zhou

doi:10.1117/12.2610585

2 March 2022 Label-free imaging of live migrasome formation using optical diffraction tomography

Yanqing Xu, Yanping He, Shiyuan Wei, Liting Duan, Renjie Zhou

Proceedings Volume 11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; 119720C (2022) https://doi.org/10.1117/12.2610585
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Migrasome is a type of recently discovered organelle that plays a vital role in the release of cytosolic contents, regulation of zebrafish embryo formation, mitochondria quality control process, etc. Fluorescence microscopy is widely used to investigate biological specimens, including migrasomes. However, the labelling of fluorescence probes not only requires additional preparation steps, but also may interfere with cellular functions and potentially result in phototoxicity, while only a limited number of labelled structures can be observed at one time. Optical diffraction tomography, as a label-free imaging technique complementary to fluorescence imaging tools, is able to characterize the biophysical properties of organelles. Here we propose to apply optical diffraction tomography for three-dimensional (3D) imaging of migrasome and monitoring its dynamics in living cells.

Conference Presentation

Citation Download Citation

Yanqing Xu, Yanping He, Shiyuan Wei, Liting Duan, and Renjie Zhou "Label-free imaging of live migrasome formation using optical diffraction tomography", Proc. SPIE 11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, 119720C (2 March 2022); https://doi.org/10.1117/12.2610585

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