Model testing capacity of global analysis of single-photon timing data

Noel Boens; Luc D. Janssens; Marcel Ameloot; Frans C. De Schryver

doi:10.1117/12.17709

1 May 1990 Model testing capacity of global analysis of single-photon timing data

Noel Boens, Luc D. Janssens, Marcel Ameloot, Frans C. De Schryver

Proceedings Volume 1204, Time-Resolved Laser Spectroscopy in Biochemistry II; (1990) https://doi.org/10.1117/12.17709
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

The performance of global (simultaneous) analysis of multiexponential fluorescence decay surfaces using reference convolution is investigated in a systematic way using simulated and experimental data sets. It is shown that the increased model discrimination ability and the more accurate parameter recovery by global analysis as compared to single curve analysis originate from combining decay traces with differing contributions of the decay components. Simultaneous analysis of decay traces in which the contributions of the components are changed as much as possible is the most beneficial. Consequently, including more decay traces in the global decay surface does not necessarily lead to a better model distinction capability. For decay surfaces collected as a function of the emission (excitation) wavelength, the decays with minimal overlap between the emission (absorption) spectra associated with the decay components will contribute the most to the improved model discrimination and parameter recovery.

Citation Download Citation

Noel Boens, Luc D. Janssens, Marcel Ameloot, and Frans C. De Schryver "Model testing capacity of global analysis of single-photon timing data", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); https://doi.org/10.1117/12.17709

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available