Resolution-enhanced quantitative phase imaging from transport of intensity equation: mixed-transfer-function

Linpeng Lu; Jiasong Sun; Yao Fan; Jialin Zhang; Qian Chen; Chao Zuo

doi:10.1117/12.2601653

24 November 2021 Resolution-enhanced quantitative phase imaging from transport of intensity equation: mixed-transfer-function

Linpeng Lu, Jiasong Sun, Yao Fan, Jialin Zhang, Qian Chen, Chao Zuo

Author Affiliations +

Proceedings Volume 12065, AOPC 2021: Optical Sensing and Imaging Technology; 1206509 (2021) https://doi.org/10.1117/12.2601653
Event: Applied Optics and Photonics China 2021, 2021, Beijing, China

Abstract

Transport of intensity equation (TIE) is an established quantitative phase imaging (QPI) method as a deter-ministic phase retrieval algorithm derived from paraxial approximation. However, this approximation limits TIE to retrieve high-resolution QPI for most biological samples. It can be overcome via the phase transfer function (PTF) method (no paraxial approximation), while it has incorrect phase in low-frequency due to the weak phase approximation. For the general microscopic specimens with thickness and small details, we present a resolution-enhanced QPI mixed-transfer-function (MTF) approach based on the spatial domain and the spatial frequency one, using phase space theory as a bridge. It is proved that the traditional TIE can obtain the accurate phase in low-frequency, but suﬀers from attenuation and blurring at high frequencies. Thus, MTF is combined with PTF to improve the maximum resolution that can be accurately reconstructed. Simulation results and experiments demonstrate that MTF can achieve high-contrast and high-resolution QPI correctly over the whole theoretical bandwidth, showing eﬃciency for phase retrieval even in slowly varying large phase objects. Besides, it is compatible with commercial microscopes without additional hardware modiﬁcation, oﬀering a ﬂexible and cost-eﬀective alternative for biomedical research and cellular investigations.

Citation Download Citation

Linpeng Lu, Jiasong Sun, Yao Fan, Jialin Zhang, Qian Chen, and Chao Zuo "Resolution-enhanced quantitative phase imaging from transport of intensity equation: mixed-transfer-function", Proc. SPIE 12065, AOPC 2021: Optical Sensing and Imaging Technology, 1206509 (24 November 2021); https://doi.org/10.1117/12.2601653

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KEYWORDS

Modulation transfer functions

Phase imaging

Phase retrieval

Deconvolution

Imaging systems

Phase transfer function

Light sources

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