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Blast-induced TBI (bTBI) is a type of traumatic brain injury generated by a shock wave that causes the head to quickly accelerate or decelerate. It has huge challenges in the diagnosis and prognosis due to its absence of significant edema and traumatic areas. The major diagnosis methods for bTBI has some defects such as time-consuming, poor specificity, low sensitivity. Thus, it is highly desirable to establish a rapid and high-sensitivity method for the detection of bTBI. Raman spectroscopy technique has been used in biochemistry due to its label-free and non-destructive. It is expected to detect and monitor the progression and regression of bTBI from molecular perspective. In this paper, the Raman spectra of the hippocampus and hypothalamus tissue were measured at 3h, 6h, 24h, 48h and 72h after mild and moderate bTBI in 2800-3000cm-1 range. The results showed both the two brain areas had a significant difference in intensity of Raman characteristic peaks at 2855, 2885 and 2934cm-1 at different time points compared with the sham group. It demonstrates that the content of the lipids and proteins have been changed in the rat hippocampus and hypothalamus after bTBI, due to the Raman peaks at 2855, 2885 and 2934cm-1 assigning to C-H stretching of lipids and protein. It infers that Raman spectroscopy technique has the potential to be a rapid and effective diagnosis and monitoring method in bTBI clinic.
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