Functional imaging of olfactory bulb and somatosensory cortex in mice using small-animal blood flow imaging platform

Ria Paul; Soumyajit Sarkar; Shruti D. Marathe; Murali K.; Susweta Das; Nixon M. Abraham; Hari M. Varma

doi:10.1117/12.2668690

7 March 2023 Functional imaging of olfactory bulb and somatosensory cortex in mice using small-animal blood flow imaging platform

Ria Paul, Soumyajit Sarkar, Shruti D. Marathe, Murali K., Susweta Das, Nixon M. Abraham, Hari M. Varma

Author Affiliations +

Proceedings Volume 12378, Dynamics and Fluctuations in Biomedical Photonics XX; 123780B (2023) https://doi.org/10.1117/12.2668690
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

A portable, compact and modular small-animal imaging platform is designed for measurement of deep tissue and superficial cerebral blood flow. The platform is integrated with optics, data acquisition unit, display unit and on-board single-board computer (SBC) that supports a Graphical User Interface (GUI). It also contains a customizable stereotaxic frame for both mice and rats for housing the animals along with provision for anesthesia, pulse oximeters and temperature probes. Functional studies have been conducted in the olfactory bulb and somatosensory cortex in mice brain using the imaging platform to measure relative cerebral blood flow (rCBF). We show longitudinal blood flow changes in the pre-cortical brain region associated with multiple odours, categorized broadly into ester, phenyl propanoid and terpenoids chemical group. A forepaw stimulation study has also been conducted to show blood flow changes in the cortical region of the brain. The surface as well as depth-wise blood flow changes due to the stimulations have been shown using Laser Speckle Contrast Imaging (LSCI) and Multi-speckle Diffuse Correlation Tomography (M-DCT) respectively.

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Ria Paul, Soumyajit Sarkar, Shruti D. Marathe, Murali K., Susweta Das, Nixon M. Abraham, and Hari M. Varma "Functional imaging of olfactory bulb and somatosensory cortex in mice using small-animal blood flow imaging platform", Proc. SPIE 12378, Dynamics and Fluctuations in Biomedical Photonics XX, 123780B (7 March 2023); https://doi.org/10.1117/12.2668690

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