Fast multi-photon brain-wide volumetric imaging and photostimulation

Lapo Turrini; Pietro Ricci; Giuseppe de Vito; Michele Sorelli; Marco Marchetti; Francesco Vanzi; Ludovico Silvestri; Francesco Saverio Pavone

doi:10.1117/12.2649824

25 April 2023 Fast multi-photon brain-wide volumetric imaging and photostimulation

Lapo Turrini, Pietro Ricci, Giuseppe de Vito, Michele Sorelli, Marco Marchetti, Francesco Vanzi, Ludovico Silvestri, Francesco Saverio Pavone

Author Affiliations +

Proceedings Volume 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; 123840B (2023) https://doi.org/10.1117/12.2649824
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

The ability to simultaneously detect and control neuronal activity grants the capacity to unravel the causal relationships behind brain processing. Nevertheless, scaling-up this technology to the whole encephalon of a vertebrate is a challenging task, and this impedes reaching a global comprehension of how the brain works. To this aim, we developed an optical system that enables fast noninvasive functional imaging of the whole brain of the zebrafish larva and, at the same time, allows us to optogenetically stimulate the activity of arbitrary sets of neurons in the volume. Our preliminary results show that with this optical system we can both consistently evoke activation of neurons in the stimulation site and identify distantly-located functionally-connected neurons placed downstream in the activated circuits. The expansibility of this concept paves the way for the brain-wide mapping of functional connectivity in the zebrafish larva.

Conference Presentation

Citation Download Citation

Lapo Turrini, Pietro Ricci, Giuseppe de Vito, Michele Sorelli, Marco Marchetti, Francesco Vanzi, Ludovico Silvestri, and Francesco Saverio Pavone "Fast multi-photon brain-wide volumetric imaging and photostimulation", Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 123840B (25 April 2023); https://doi.org/10.1117/12.2649824

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available