A 3D printed microfluidic PCR device towards detecting SARS-CoV-2

Kristi Shaka; Kent Jones; Aaron Putzke; Philip Measor

doi:10.1117/12.2648519

16 March 2023 A 3D printed microfluidic PCR device towards detecting SARS-CoV-2

Kristi Shaka, Kent Jones, Aaron Putzke, Philip Measor

Proceedings Volume 12387, Optical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics; 123870C (2023) https://doi.org/10.1117/12.2648519
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

A 3D printed (3DP) microfluidic polymerase chain reaction (PCR) device was demonstrated by detecting synthetic SARS-CoV-2 at 106 copies/μL. The microfluidic device was fabricated using stereolithography 3DP and had a reaction volume of ~22 nL. The microdevice showed PCR amplification with 85 base synthetic ssDNA targets and primers designed for a SARS-CoV-2-specific region. The device was 2.5 times faster compared to a qPCR instrument with >60,000 times smaller reagent volume. The 3DP microdevice is a promising technology to significantly reduce the manufacturing costs of microfluidic devices that could be used towards point-of-care applications.

Conference Presentation

Citation Download Citation

Kristi Shaka, Kent Jones, Aaron Putzke, and Philip Measor "A 3D printed microfluidic PCR device towards detecting SARS-CoV-2", Proc. SPIE 12387, Optical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics, 123870C (16 March 2023); https://doi.org/10.1117/12.2648519

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