Hydrodynamics mediated interactions between live active rotors in dual optical tweezers

Ashwini V. Bhat; Naveena C. S.; Sharath Ananthamurthy

doi:10.1117/12.3008383

20 September 2023 Hydrodynamics mediated interactions between live active rotors in dual optical tweezers

Ashwini V. Bhat, Naveena C. S., Sharath Ananthamurthy

Proceedings Volume 12606, Optical Manipulation and Structured Materials Conference; 126061F (2023) https://doi.org/10.1117/12.3008383
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

We report on the interactions between internally driven pairs of active rotors in a dual optical tweezer. The active rotor is Bacillus subtilis, a wild-type, gram- positive bacterium that uses flagellar rotation for motility. A pair of bacteria are held at different distances and their respective flagellar rotations are studied through the durations of their approach and retraction from each other. The aim of our work is to investigate the nature of the interactions between two active confined rotors trapped in their pristine form. We find, that the frequency of the rotating flagella decreases in both confined bacteria on approaching each other and increases when retracted from each other. In other words, the flagellar rotations of a bacterium slow down while in the presence of a nearby neighbor and speeds up as the neighbor retreats. Our results show a similar trend as when compared to free swimming bacteria wherein they avoid each other on approach through modulation of their rotating flagella. We investigate through this setup the hydrodynamics mediated coupling between two such active rotors.

Citation Download Citation

Ashwini V. Bhat, Naveena C. S., and Sharath Ananthamurthy "Hydrodynamics mediated interactions between live active rotors in dual optical tweezers", Proc. SPIE 12606, Optical Manipulation and Structured Materials Conference, 126061F (20 September 2023); https://doi.org/10.1117/12.3008383

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Bacteria

Optical tweezers

Dichroic mirrors

Scattered light

Video microscopy

Objectives

Physics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years