Structural and mechanical insights into DNA and chromosomes under strain using optical tweezers and fluorescence microscopy

Erwin J. G. Peterman

doi:10.1117/12.2682173

5 October 2023 Structural and mechanical insights into DNA and chromosomes under strain using optical tweezers and fluorescence microscopy

Erwin J. G. Peterman

Author Affiliations +

Proceedings Volume 12649, Optical Trapping and Optical Micromanipulation XX; 1264902 (2023) https://doi.org/10.1117/12.2682173
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States

Abstract

In our lab, we have developed the combination of optical tweezers and single-molecule fluorescence microscopy as a powerful tool to study biomolecules and biomolecular complexes. Combining these two technologies allows holding a sample molecule or complex, extending or deforming it, and measuring forces acting on it, while, at the same time, visualizing it with single-molecule sensitivity. We have applied this approach to different biological systems, including DNA, whole chromosomes, cytoskeletal components and membranes. Here, I will explain the concept of the technology, its potential and limitations. I will explain the technology by highlighting its application to our research on the mechanical properties of DNA and force-induced conformational transitions. I will also discuss our latest breakthroughs in applying this technology to intact mitotic human chromosomes, which provide novel insights in the mechanical properties of chromosomes and the way they are condensed in mitosis.

Conference Presentation

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Erwin J. G. Peterman "Structural and mechanical insights into DNA and chromosomes under strain using optical tweezers and fluorescence microscopy", Proc. SPIE 12649, Optical Trapping and Optical Micromanipulation XX, 1264902 (5 October 2023); https://doi.org/10.1117/12.2682173

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