Optogenetic stimulation and fast label-free wide field video microscopy for the investigation of excitation wavefronts in in vitro cardiac tissue

Adam T. Pierce; Felix Schmieder; Lars Büttner; Wouter Derks; Olaf Bergmann; Jürgen W. Czarske

doi:10.1117/12.3003511

13 March 2024 Optogenetic stimulation and fast label-free wide field video microscopy for the investigation of excitation wavefronts in in vitro cardiac tissue

Adam T. Pierce, Felix Schmieder, Lars Büttner, Wouter Derks, Olaf Bergmann, Jürgen W. Czarske

Author Affiliations +

Proceedings Volume 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024; 1281910 (2024) https://doi.org/10.1117/12.3003511
Event: SPIE BiOS, 2024, San Francisco, California, United States

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Abstract

In hearts, self-replicating reentrant spiral waves can cause deadly tachycardia. In vitro cardiac tissue models can benefit both fundamental research and patient-specific disease modeling. Here, cardiac optogenetics allows damage-free control of tissue activity. We present a system for digital control and observation of excitation wavefronts in human stem-cell-derived cardiomyocytes expressing f-ChRimson-YFP. Holographic light shaping enables patterned illumination, observing the 5x5 mm² sample at 250Hz frame rate. A fast data evaluation scheme accesses spatially resolved stimulus-induced sample activity and the propagation of action potential wavefronts in in vitro cell cultures. Experiments with varied illumination patterns control wavefront direction and timing, paving the way for patient-specific disease modeling.

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Adam T. Pierce, Felix Schmieder, Lars Büttner, Wouter Derks, Olaf Bergmann, and Jürgen W. Czarske "Optogenetic stimulation and fast label-free wide field video microscopy for the investigation of excitation wavefronts in in vitro cardiac tissue", Proc. SPIE 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024, 1281910 (13 March 2024); https://doi.org/10.1117/12.3003511

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