Time dependent quantification of paired agent imaging in head and neck squamous cell carcinomas: determining the effects of imaging agent concentrations on binding potential

Sanjana Pannem; Yichen Feng; Sassan Hodge; Eunice Chen; Jonathan Elliott; Kenneth M. Tichauer; Keith Paulsen; Kimberley S. Samkoe

doi:10.1117/12.3002812

12 March 2024 Time dependent quantification of paired agent imaging in head and neck squamous cell carcinomas: determining the effects of imaging agent concentrations on binding potential

Sanjana Pannem, Yichen Feng, Sassan Hodge, Eunice Chen, Jonathan Elliott, Kenneth M. Tichauer, Keith Paulsen, Kimberley S. Samkoe

Author Affiliations +

Proceedings Volume 12825, Molecular-Guided Surgery: Molecules, Devices, and Applications X; 128250C (2024) https://doi.org/10.1117/12.3002812
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Paired-Agent Imaging (PAI) is a quantitative fluorescence imaging technique that estimates the drug target concentration. It involves the co-administration of a targeted and an untargeted imaging probe to correct for nonspecific uptake and to quantify the available receptor concentration, known as the binding potential. PAI has been demonstrated in a pre-clinical setting using a 1:1 molar concentration of the targeted, ABY-029, and untargeted, IRDYE 680LT, imaging agent. However, the effects of different molar concentrations of imaging agents on the binding potential have not been studies thus far. In this study, we examined this relationship in tissue-mimicking liquid phantoms with varying molar concentration ratios. The phantom fluorescence was measured using the Pearl Imaging system and then the binding potential was quantified using MATLAB. We determined that the binding potential remains stable across concentration but increases for dye ratios where the targeted dye is higher.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Sanjana Pannem, Yichen Feng, Sassan Hodge, Eunice Chen, Jonathan Elliott, Kenneth M. Tichauer, Keith Paulsen, and Kimberley S. Samkoe "Time dependent quantification of paired agent imaging in head and neck squamous cell carcinomas: determining the effects of imaging agent concentrations on binding potential", Proc. SPIE 12825, Molecular-Guided Surgery: Molecules, Devices, and Applications X, 128250C (12 March 2024); https://doi.org/10.1117/12.3002812

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