Polarization-sensitive Brillouin microscopy for corneal imaging

Hongyuan Zhang; Giuliano Scarcelli; James Bradley Randleman

doi:10.1117/12.3003378

12 March 2024 Polarization-sensitive Brillouin microscopy for corneal imaging

Hongyuan Zhang, Giuliano Scarcelli, James Bradley Randleman

Proceedings Volume 12844, Optical Elastography and Tissue Biomechanics XI; 1284404 (2024) https://doi.org/10.1117/12.3003378
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Due to anisotropic fibril orientations across the cornea, different phase retardation is superimposed on the reflection, depending on the imaging area. As polarized light is commonly used in Brillouin microscopy to maximize the collection of the scattered light, polarization mismatch can strongly reduce the intensity of Brillouin signal, making the deep stroma undetectable. Therefore, a quarter wave plate was added to compensate corneal phase retardation according to the intensity of Brillouin scattering, aiming at getting a clear depth-dependent profile of Brillouin shifts. The compensated Brillouin profile showed similarity with profiles measured at points with limited phase retardation impact, 60 MHz smaller than the profile without compensation.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Hongyuan Zhang, Giuliano Scarcelli, and James Bradley Randleman "Polarization-sensitive Brillouin microscopy for corneal imaging", Proc. SPIE 12844, Optical Elastography and Tissue Biomechanics XI, 1284404 (12 March 2024); https://doi.org/10.1117/12.3003378

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