Our high-speed multimodal imaging platform combines stimulated Raman Scattering (SRS), multiphoton fluorescence (MPF), and second harmonic generation (SHG) to explore metabolic activities of biomolecules in cells and tissues. Using the A-PoD algorithm, we achieve super-resolution imaging, while deuterium oxide probing enhances SRS imaging. The Correlation Coefficient Mapping (CoCoMap) technique helps understand coordination and regulation of metabolic activities. This tool enables early disease detection, prognosis, and therapeutic assessment, providing valuable insights into aging and metabolic diseases. Our approach revolutionizes metabolism visualization, advancing knowledge of biological processes and their implications in health and disease.
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