Simulating surface plasmon resonance for enhanced imaging of C. elegans

Karen Regules-Medel; Veeren Chauhan; Silvia Maggi; Michael Somekh; Sidahmed Abayzeed

doi:10.1117/12.3002479

12 March 2024 Simulating surface plasmon resonance for enhanced imaging of C. elegans

Karen Regules-Medel, Veeren Chauhan, Silvia Maggi, Michael Somekh, Sidahmed Abayzeed

Proceedings Volume 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; 128540U (2024) https://doi.org/10.1117/12.3002479
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

This manuscript reports the application of Surface Plasmon Resonance imaging for analysing C. elegans nematodes. Our aim is to utilise this simulation to examine the effect of refractive index and geometry of C. elegans in order to gain deeper insights into the nematode's biological features. Employing Fresnel equations and the Transfer-Matrix Method, we investigated these aspects at the sensor-nematode interface, which is part of a multilayer model consisting of a 50 nm gold thin film deposited on a semi-infinite glass substrate. The nematode is immobilised on the thin film via an agarose sheet that forms a microfluidics channel filled with phosphate-buffered saline (PBS). The worm is modelled as an ellipsoidal crosssection allowing us to varying ellipse parameters and adherence profile on the gold thin film. Our findings illustrate the effects of the gap between the gold film and the worm, the geometry of the worm’s body, and its refractive index on SPR imaging. Our results indicate variations in the SPR response due to the geometry of both agarose and worm tissue with no observed response beyond the penetration depth of the SPR sensor. The observed range of SPR angle change was from 0 to 7°, with the most substantial changes noted in the worm area. This research highlights the potential for employing highly sensitive, label-free SPR microscopy techniques in the biological imaging of the C. elegans cuticle.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Karen Regules-Medel, Veeren Chauhan, Silvia Maggi, Michael Somekh, and Sidahmed Abayzeed "Simulating surface plasmon resonance for enhanced imaging of C. elegans", Proc. SPIE 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, 128540U (12 March 2024); https://doi.org/10.1117/12.3002479

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