Pre-processing for precise super-resolution multimodal image analysis

Hongje Jang; Zhi Li; Fenja Bahrenburg; Ellen Ackerstaff; Jason A. Koutcher; Lingyan Shi

doi:10.1117/12.3006012

12 March 2024 Pre-processing for precise super-resolution multimodal image analysis

Hongje Jang, Zhi Li, Fenja Bahrenburg, Ellen Ackerstaff, Jason A. Koutcher, Lingyan Shi

Proceedings Volume 12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; 1285505 (2024) https://doi.org/10.1117/12.3006012
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We have developed a novel methodology to capture images of various biomolecules at a resolution surpassing the traditional diffraction limit of optical microscopy. By harnessing a multimodal imaging platform that combines stimulated Raman scattering (SRS), multiphoton fluorescence (MPF), and second harmonic generation (SHG), together with sophisticated image deconvolution algorithms, we have successfully generated super-resolution images that reveal the details of biomolecular metabolism. These images enable us to explore the intricate associations between metabolic activities and the spatial distribution of metabolites within breast cancer tissues. To enhance the accuracy of this measurement technique, in this study, we designed a pre-processing workflow that incorporates both denoising and drift correction processes. Our cutting-edge, nonlinear multimodal imaging approach, when applied in a super-resolution context with new workflow, holds significant promise for advancing early detection of breast cancer, prognostication, evaluation of therapeutic outcomes, and deepening our mechanistic understanding of diseases.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Hongje Jang, Zhi Li, Fenja Bahrenburg, Ellen Ackerstaff, Jason A. Koutcher, and Lingyan Shi "Pre-processing for precise super-resolution multimodal image analysis", Proc. SPIE 12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, 1285505 (12 March 2024); https://doi.org/10.1117/12.3006012

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