Molecular identification of second harmonic generation (SHG) sources in mouse brain by multimodal imaging with ultra-broadband multiplex coherent anti Stokes Raman scattering (CARS)

Yusuke Murakami; Ryosuke Oketani; Philippe Leproux; Mustuo Nuriya; Sakiko Honjoh; Kano Hideaki

doi:10.1117/12.2692336

12 March 2024 Molecular identification of second harmonic generation (SHG) sources in mouse brain by multimodal imaging with ultra-broadband multiplex coherent anti Stokes Raman scattering (CARS)

Yusuke Murakami, Ryosuke Oketani, Philippe Leproux, Mustuo Nuriya, Sakiko Honjoh, Kano Hideaki

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Proceedings Volume 12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; 128550C (2024) https://doi.org/10.1117/12.2692336
Event: SPIE BiOS, 2024, San Francisco, California, United States

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Abstract

Second harmonic generation imaging is a powerful tool for visualizing molecular structures in living organisms without the need for exogenous dyes. However, SHG signal lacks molecular specificity in identifying the source. This study aimed at molecular identification of SHG sources in the mouse brain using a multimodal imaging technique combining SHG and multiplex coherent anti Stokes Raman scattering (CARS) spectroscopy. We performed multimodal imaging in two different regions, the surface and dentate gyrus of the brain tissue. For the brain surface, the SHG signal was recognized through CARS spectrum analysis, indicating its origin in collagen. In the dentate gyrus, CARS images did not unveil corresponding molecular origins; however, morphologically, the SHG signal likely originated from Rootletin within neurons. Overall, Multimodal imaging approach to molecular identification of SHG has the potential to contribute to a comprehensive understanding of the molecular and structural features of the mouse brain. These findings advance label-free imaging techniques and have implications for brain tissue analysis and functional mapping research.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Yusuke Murakami, Ryosuke Oketani, Philippe Leproux, Mustuo Nuriya, Sakiko Honjoh, and Kano Hideaki "Molecular identification of second harmonic generation (SHG) sources in mouse brain by multimodal imaging with ultra-broadband multiplex coherent anti Stokes Raman scattering (CARS)", Proc. SPIE 12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, 128550C (12 March 2024); https://doi.org/10.1117/12.2692336

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