Light-scattering in absorption spectra: a literature survey of examples and corrections

Masahiko Taniguchi; Jonathan S. Lindsey

doi:10.1117/12.3000407

13 March 2024 Light-scattering in absorption spectra: a literature survey of examples and corrections

Masahiko Taniguchi, Jonathan S. Lindsey

Proceedings Volume 12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV; 128620B (2024) https://doi.org/10.1117/12.3000407
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Absorption spectroscopy is a simple yet robust method for qualitative and quantitative analysis. The technique is most commonly applied for molecules in solution that absorb in the ultraviolet (UV), visible (Vis), and/or near-infrared (NIR) spectral region. The presence of the particles of size comparable to or greater than the wavelength of light can cause scattering. The contribution from light scattering can overwhelm intrinsic absorption especially in the shorter wavelength, UV regions, which impedes accurate analysis. The problem of light scattering is common in absorption spectroscopy. To compensate for the alterations caused by light scattering, various correction methods have been applied over the years. The chief correction methods are based on Rayleigh or Mie light-scattering theory. Displayed here are >40 spectra drawn from spectroscopy and photochemistry journals published over 7 decades (since the 1950s) that illustrate the lightscattering problem. The displays include >25 spectra beset by light scattering and 15 examples wherein spectral corrections have been applied. The present survey of the scope of the light-scattering problem in absorption spectroscopy (200–1000 nm) serves as a prelude to the development of a systematic calculational module for spectral correction.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Masahiko Taniguchi and Jonathan S. Lindsey "Light-scattering in absorption spectra: a literature survey of examples and corrections", Proc. SPIE 12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV, 128620B (13 March 2024); https://doi.org/10.1117/12.3000407

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available