Detection of microorganisms in drinking water using fluorescence spectroscopy

Jörgen Gladh; Julia Hedborg; Per Jonsson; Fredrik Kullander; Per Ola Andersson; Alexandra C. Johansson; Lars Landström

doi:10.1117/12.3014251

7 June 2024 Detection of microorganisms in drinking water using fluorescence spectroscopy

Jörgen Gladh, Julia Hedborg, Per Jonsson, Fredrik Kullander, Per Ola Andersson, Alexandra C. Johansson, Lars Landström

Proceedings Volume 13056, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXV; 130560K (2024) https://doi.org/10.1117/12.3014251
Event: SPIE Defense + Commercial Sensing, 2024, National Harbor, Maryland, United States

Abstract

To guarantee safe supply of drinking water, there is a need for fast, sensitive and robust techniques for early warning of potentially pathogenic microorganisms. An optical measurement system has been developed to spectrally study the UVinduced autofluorescence from single microorganisms in a water flow. Particles in the flow are detected with a continuouswave laser whereby an ultraviolet laser pulse is fired and a spectrometer measures emitted fluorescence. Suspensions of Cryptosporidium parvum (Crypto), Bacillus atrophaeus (BG, a non-pathogenic simulant bacterial spore) and Escherichia coli (Ecoli), in distilled and tap water have been examined. The results from single particle measurements are compared to fluorescence emission spectra captured on suspensions with a fluorescence spectrometer and the strength and variability of single organism spectra, with respect to detection applications, is also investigated.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Jörgen Gladh, Julia Hedborg, Per Jonsson, Fredrik Kullander, Per Ola Andersson, Alexandra C. Johansson, and Lars Landström "Detection of microorganisms in drinking water using fluorescence spectroscopy", Proc. SPIE 13056, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXV, 130560K (7 June 2024); https://doi.org/10.1117/12.3014251

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