Suitability of synchrotron-radiation phase-contrast tomography for imaging of inner ear of harbor porpoise

Laura Rojas; Julian Moosmann; Felix Beckmann; Louise Moysan; Ursula Siebert; Lonneke IJsseldijk; Imke A. K. Fiedler; Björn Busse; Maria Morell

doi:10.1117/12.3029198

31 October 2024 Suitability of synchrotron-radiation phase-contrast tomography for imaging of inner ear of harbor porpoise

Laura Rojas, Julian Moosmann, Felix Beckmann, Louise Moysan, Ursula Siebert, Lonneke IJsseldijk, Imke A. K. Fiedler, Björn Busse, Maria Morell

Author Affiliations +

Proceedings Volume 13152, Developments in X-Ray Tomography XV; 1315223 (2024) https://doi.org/10.1117/12.3029198
Event: Optical Engineering + Applications, 2024, San Diego, California, United States

Conference Poster

Abstract

Synchrotron-radiation phase-contrast tomography is a non-invasive technique that allows high-resolution imaging of soft structures. We present the first results of the cochlea (inner ear) of a harbor porpoise (Phocoena phocoena). Samples were analyzed at a high spatial resolution using synchrotron-radiation propagation-based phase-contrast microtomography at the high-energy materials science beamline (HEMS) P07, operated by Hereon, at the storage ring PETRA III at DESY. Since these samples are larger than the x-ray beam profile, a scanning and projection-based stitching approach was employed. Stitching of the acquired raw data sets resulted in centimeter-sized reconstructions with 2.54μm voxel size and volumes ranging from 280GB to 5.2TB. Synchrotron-radiation phase-contrast tomography proved to be a suitable technique to image the spiral ganglion cells and other structures of the inner ear of a harbor porpoise.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Laura Rojas, Julian Moosmann, Felix Beckmann, Louise Moysan, Ursula Siebert, Lonneke IJsseldijk, Imke A. K. Fiedler, Björn Busse, and Maria Morell "Suitability of synchrotron-radiation phase-contrast tomography for imaging of inner ear of harbor porpoise", Proc. SPIE 13152, Developments in X-Ray Tomography XV, 1315223 (31 October 2024); https://doi.org/10.1117/12.3029198

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