3D fluorescence image analysis algorithms for quantifying cytotoxic behavior of CAR-T cells

Zhaofei Wang; Wentian Si; Peng Fei

doi:10.1117/12.3046025

13 December 2024 3D fluorescence image analysis algorithms for quantifying cytotoxic behavior of CAR-T cells

Zhaofei Wang, Wentian Si, Peng Fei

Proceedings Volume 13503, AOPC 2024: Biomedical Optics; 1350302 (2024) https://doi.org/10.1117/12.3046025
Event: Applied Optics and Photonics China 2024 (AOPC2024), 2024, Beijing, China

Abstract

One of the issues impeding the application of chimeric antigen receptor T-cell (CAR-T) therapy is that some patients have T cell dysfunction, making treatment ineffective. To objectively screen these patients, we developed 3D fluorescence image analysis algorithm for quantifying cytotoxic behavior of CAR-T cells. Indicators include the area of immune synapses (IS), the actin depletion coefficient of IS, the actin migration rate, and the centrosome polarization angle. Our results demonstrate that these indicators are capable of differentiate CAR-T cell function.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Zhaofei Wang, Wentian Si, and Peng Fei "3D fluorescence image analysis algorithms for quantifying cytotoxic behavior of CAR-T cells", Proc. SPIE 13503, AOPC 2024: Biomedical Optics, 1350302 (13 December 2024); https://doi.org/10.1117/12.3046025

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