Digital imaging of Giemsa-banded human chromosomes: eigenanalysis and the Fourier phase reconstruction

Zeljko Jericevic; Loris McGavran; Louis C. Smith

doi:10.1117/12.44142

1 May 1991 Digital imaging of Giemsa-banded human chromosomes: eigenanalysis and the Fourier phase reconstruction

Zeljko Jericevic, Loris McGavran, Louis C. Smith

Proceedings Volume 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences; (1991) https://doi.org/10.1117/12.44142
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

The new methodology of chromosome analysis based on eigenanalysis and iterative Fourier synthesis has been developed. The approach is inspired by the analysis developed in electron microscopy of particles, and has been modified to address particular problems of chromosome analysis. Preliminary results on data sets containing 40-80 images for each of the human chromosomes indicate that this methodology provides an improvement of chromosome band resolution and potentially can provide cytogeneticist with some new insights. The proposed procedure is a novel approach in chromosome analysis and represents a significant contribution to quantitative cytogenetics. It opens the possibility of identifying defects in chromosome banding pattern automatically.

Citation Download Citation

Zeljko Jericevic, Loris McGavran, and Louis C. Smith "Digital imaging of Giemsa-banded human chromosomes: eigenanalysis and the Fourier phase reconstruction", Proc. SPIE 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences, (1 May 1991); https://doi.org/10.1117/12.44142

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