Electrically Induced Fluorescence Changes For Characterising Macromolecules

P. J. Ridler; B. R. Jennings

doi:10.1117/12.965503

25 July 1979 Electrically Induced Fluorescence Changes for Characterising Macromolecules

P. J. Ridler, B. R. Jennings

Proceedings Volume 0164, 4th European Electro-Optics Conf; (1979) https://doi.org/10.1117/12.965503
Event: Fourth European Electro-Optics Conference, 1978, Utrecht, Netherlands

Abstract

Fluorescence involves the absorption and reemission of light, in which incident linearly polarised radiation is generally depolarised depending upon the directions in space of the corresponding molecular transition moments. An applied electric field imposes order on macromolecules in dilute solution and the polarised components of the fluorescence change. An apparatus is described in which the changes in the four polarised components are measured for solutions of dye-tagged nucleic acids, polymers, pigment particles and liquid crystals. Using pulsed electric fields, information on the nature of dye binding and the size of the macromolecules is obtained.

Citation Download Citation

P. J. Ridler and B. R. Jennings "Electrically Induced Fluorescence Changes for Characterising Macromolecules", Proc. SPIE 0164, 4th European Electro-Optics Conf, (25 July 1979); https://doi.org/10.1117/12.965503

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