Vesicle size measurement by static light scattering: a Fourier cosine transform approach

Jianhong Wang; F. Ross Hallett

doi:10.1117/12.210031

30 May 1995 Vesicle size measurement by static light scattering: a Fourier cosine transform approach

Jianhong Wang, F. Ross Hallett

Author Affiliations +

Proceedings Volume 2389, Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation; (1995) https://doi.org/10.1117/12.210031
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Based on the Rayleigh-Gans-Debye thin shell approximation, a fast Fourier cosine transform method was developed to retrieve vesicle size distributions directly from the light scattering measurement. The method was tested numerically mainly on the scattering data generated by the exact Mie solution for isotropic vesicles. Monomodal and bimodal Gaussian distributions were used to represent vesicles with radii ranging from 0.05 micrometers to 0.5 micrometers . The recovered distributions were in good aggreement with the original distributions. Peak positions were recovered within 10% of the original ones, even when 20% random error was added to the simulated data. Noise tolerance of this method was much higher for large vesicles than for small vesicles. The effect of the refractive index of the medium was also investigated using the simulated data. To study the effect of the optical anisotropy of the membrane on the recovery, the exact Mie solution for anisotropic hollow spheres was used to simulate scattered intensity data. The recovered distributions in this case were slightly shifted towards small radii from the original ones.

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Jianhong Wang and F. Ross Hallett "Vesicle size measurement by static light scattering: a Fourier cosine transform approach", Proc. SPIE 2389, Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation, (30 May 1995); https://doi.org/10.1117/12.210031

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KEYWORDS

Light scattering

Mie scattering

Scattering

Refractive index

Fourier transforms

Anisotropy

Optical testing

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