Fiber optic based fluorescence detection system for in vivo studies of exogenous chromophore pharmacokinetics

Daniel R. Doiron; J. Brian Dunn; W. L. Mitchell; Brian K. Dalton; Greta M. Garbo; Jon A. Warner

doi:10.1117/12.208418

1 May 1995 Fiber optic-based fluorescence detection system for in vivo studies of exogenous chromophore pharmacokinetics

Daniel R. Doiron, J. Brian Dunn, W. L. Mitchell, Brian K. Dalton, Greta M. Garbo, Jon A. Warner

Author Affiliations +

Proceedings Volume 2396, Biomedical Optoelectronic Instrumentation; (1995) https://doi.org/10.1117/12.208418
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

The detection and quantification of the concentration of exogenous chromophores in-vivo by their fluorescence is complicated by many physical and geometrical parameters. Measurement of such signals is advantageous in determining the pharmacokinetics of photosensitizers such as those used in photodynamic therapy (PDT) or to assist in the diagnosis of tissue histological state. To overcome these difficulties a ratio based fiber optic contact fluorometer has been developed. This fluorescence detection system (FDS) uses the ratio of the fluorescence emission peak of the exogenous chromophore to that of endogenous chromophores, i.e. autofluorescence, to correct for a variety of parameters affecting the magnitude of the measured signals. By doing so it also minimizes the range of baseline measurements prior to exogenous drug injection, for various tissue types. Design of the FDS and results of its testing in animals and patients using the second generation photosensitizer Tin ethyletiopurpurin (SnET2) are presented. These results support the feasibility and usefulness of the Ratio FDS system.

Citation Download Citation

Daniel R. Doiron, J. Brian Dunn, W. L. Mitchell, Brian K. Dalton, Greta M. Garbo, and Jon A. Warner "Fiber optic-based fluorescence detection system for in vivo studies of exogenous chromophore pharmacokinetics", Proc. SPIE 2396, Biomedical Optoelectronic Instrumentation, (1 May 1995); https://doi.org/10.1117/12.208418

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