Design of a dynamic dermofluorometer for the measurement of skin perfusion

Anjin Zhang; Deborah Oh; Richard Magin

doi:10.1117/12.216871

18 August 1995 Design of a dynamic dermofluorometer for the measurement of skin perfusion

Anjin Zhang, Deborah Oh, Richard Magin

Proceedings Volume 2622, Optical Engineering Midwest '95; (1995) https://doi.org/10.1117/12.216871
Event: Optical Engineering Midwest '95, 1995, Chicago, IL, United States

Abstract

The design of a two-channel dynamic dermofluorometer to monitor skin perfusion using a fluorescent dye is reported in this paper. The hardware of this system consists of an optical module (flash lamp, optical fibers, dichroic beamsplitters, and optical wavelength filters), an electrical module, and a Macintosh computer. This system is controlled by software written using LabVIEW and MPW C running with the support of a Lab-NB multifunction board. This instrument is capable of real-time data acquisition, curve fitting, and display. Two fluorescent dyes, carboxyfluorescein and sodium fluorescein, were used in prelimiary animal studies (6 rats). Each dye was injected intravenously and its signal collected by an optical fiber bundle (diameter 3.2 mm) placed on the skin. The washin time constants were similar for both dyes (approximately 5 min.), but sodium fluorescein exhibited a much slower clearance time constant (110 min. vs. 65 min.). The instrument was tested on four normal human volunteers. The washin time constant of sodium fluorescein (1 mg/kg) for two skin sites on the forearm was measured on each subject (average 2-3 min.). These results demonstrate the potential of this instrument for performing dynamic fluorescence measurements. Future studies on diabetic patients are planned at the Danville, Illinois VA Medical Center.

Citation Download Citation

Anjin Zhang, Deborah Oh, and Richard Magin "Design of a dynamic dermofluorometer for the measurement of skin perfusion", Proc. SPIE 2622, Optical Engineering Midwest '95, (18 August 1995); https://doi.org/10.1117/12.216871

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available