Single crayfish neuron as a new test-object for search and examination of PDT photosensitizers

Anatoly B. Uzdensky; Olga Y. Kutko; Natalya V. Pasikova

doi:10.1117/12.231006

31 January 1996 Single crayfish neuron as a new test-object for search and examination of PDT photosensitizers

Anatoly B. Uzdensky, Olga Y. Kutko, Natalya V. Pasikova

Proceedings Volume 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities; (1996) https://doi.org/10.1117/12.231006
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

An isolated crayfish stretch receptor neuron was used as a new test-object for cytophysiological study of various photosensitizers. This large cell is very suitable for complex electrophysiological and cytological investigation. It generates spikes with a nearly constant frequency, and dynamics of impulse activity shifts under the laser irradiation may be precisely studied at this stable background. The experimental procedure was as follows: 30 min control spike frequency registration, 30 min neuron staining, He-Ne-laser irradiation with continuous registration of cell response dynamics. The typical response of photosensitized neuron to laser irradiation was impulse activity acceleration after some latency and then irreversible block of spike generation. Dependencies of spike frequency acceleration and neuron lifetime on photosensitizer concentration allowed us to compare different photosensitizer efficiencies. As the first set of photosensitizers methylene blue, janus green, bengal rose, and chlorine e6, were studied. Chlorine e6 was the most potent photosensitizer among them. Such an approach provides evaluation of both: initial threshold alteration in cell membrane and cytotoxic events leading to the cell death.

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Anatoly B. Uzdensky, Olga Y. Kutko, and Natalya V. Pasikova "Single crayfish neuron as a new test-object for search and examination of PDT photosensitizers", Proc. SPIE 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities, (31 January 1996); https://doi.org/10.1117/12.231006

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