Confocal scanning beam laser microscope/macroscope: applications in fluorescence

Arthur E. Dixon; Savvas Damaskinos; Alfonso Ribes

doi:10.1117/12.236197

25 March 1996 Confocal scanning beam laser microscope/macroscope: applications in fluorescence

Arthur E. Dixon, Savvas Damaskinos, Alfonso Ribes

Author Affiliations +

Proceedings Volume 2705, Fluorescence Detection IV; (1996) https://doi.org/10.1117/12.236197
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

A new confocal scanning beam laser microscope/macroscope is described that combines the rapid scan of a scanning beam laser microscope with the large specimen capability of a scanning stage microscope. This instrument combines an infinity-corrected confocal scanning laser microscope with a scanning laser macroscope that uses a telecentric f*(Theta) laser scan lens to produce a confocal imaging system with a resolution of 0.25 microns at a field of view of 25 microns and 5 microns at a field of view of 75,000 microns. The frame rate is 5 seconds per frame for a 512 by 512 pixel image, and 25 seconds for a 2048 by 2048 pixel image. Applications in fluorescence are discussed that focus on two important advantages of the instrument over a confocal scanning laser microscope: an extremely wide range of magnification, and the ability to image very large specimens. Examples are presented of fluorescence and reflected-light images of high quality printing, fluorescence images of latent fingerprints, packaging foam, and confocal autofluorescence images of a cricket.

Citation Download Citation

Arthur E. Dixon, Savvas Damaskinos, and Alfonso Ribes "Confocal scanning beam laser microscope/macroscope: applications in fluorescence", Proc. SPIE 2705, Fluorescence Detection IV, (25 March 1996); https://doi.org/10.1117/12.236197

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