Autofluorescence properties of colonic mucosa: dependence on excitation wavelength

Giovanni F. Bottiroli; Piera Balzarini; Anna Cleta Croce; Raffaella Rizzo; Marco Dal Fante; Renato Marchesini

doi:10.1117/12.260639

11 December 1996 Autofluorescence properties of colonic mucosa: dependence on excitation wavelength

Giovanni F. Bottiroli, Piera Balzarini, Anna Cleta Croce, Raffaella Rizzo, Marco Dal Fante, Renato Marchesini

Proceedings Volume 2927, Optical and Imaging Techniques for Biomonitoring II; (1996) https://doi.org/10.1117/12.260639
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

The dependence of autofluorescence properties on the excitation wavelength was studied on colon tissue, comparing neoplastic with non-neoplastic tissues. Studies were performed both on tissue sections and on whole tissue from biopsies of surgical resection, using microscopic and optic fiber devices, respectively. The excitation wavelengths 366, 405 and 436 nm, corresponding to emission lines of Mercury lamp, were considered. The results indicate that the emission intensity and spectral shape of autofluorescence of both the histological components of tissue and of the whole tissue are affected by the excitation wavelength to an extent varying with tissue condition. Excitation at 366 nm enhances the differences between non-neoplastic and neoplastic tissues in the spectral region mainly related to the tissue metabolism (NADH); excitation at 436 nm favors the differences attributable to the tissue alteration arising from tumor host-response. The findings provide useful information for optimizing autofluorescence-based technique in tumor diagnosis.

Citation Download Citation

Giovanni F. Bottiroli, Piera Balzarini, Anna Cleta Croce, Raffaella Rizzo, Marco Dal Fante, and Renato Marchesini "Autofluorescence properties of colonic mucosa: dependence on excitation wavelength", Proc. SPIE 2927, Optical and Imaging Techniques for Biomonitoring II, (11 December 1996); https://doi.org/10.1117/12.260639

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