Image analysis of cell natural fluorescence: diagnostic applications in haematology

Monica Monici; Giovanni Agati; Piero Mazzinghi; Franco Fusi; Pietro Antonio Bernabei; Stefano Landini; Pierluigi Rossi Ferrini; Riccardo Pratesi

doi:10.1117/12.259969

26 November 1996 Image analysis of cell natural fluorescence: diagnostic applications in haematology

Monica Monici, Giovanni Agati, Piero Mazzinghi, Franco Fusi, Pietro Antonio Bernabei, Stefano Landini, Pierluigi Rossi Ferrini, Riccardo Pratesi

Author Affiliations +

Proceedings Volume 2928, Biomedical Systems and Technologies; (1996) https://doi.org/10.1117/12.259969
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Haematic cells, excited with radiation of suitable wavelength, give rise to a natural fluorescence (NF) emission. This paper investigates NF to develop new techniques for applications in both basic research and medical diagnostics. Results show that the cell populations examined exhibit peculiar emission bands. The intracellular fluorescence pattern reveals that flouresence is mainly located at cytoplasmic-level, thus related to the metabolic processes of the cells. The photophysical properties of cells appear different among the normal populations and between normal and leukaemic ones. Therefore the recognition of the various cellular elements, according to their fluorescence emission, is possible.

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Monica Monici, Giovanni Agati, Piero Mazzinghi, Franco Fusi, Pietro Antonio Bernabei, Stefano Landini, Pierluigi Rossi Ferrini, and Riccardo Pratesi "Image analysis of cell natural fluorescence: diagnostic applications in haematology", Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); https://doi.org/10.1117/12.259969

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