Far-ultraviolet absorption spectra of porcine and human corneas

Annamarie Lembares; Xin-Hua Hu; Gerhard W. Kalmus

doi:10.1117/12.275123

26 May 1997 Far-ultraviolet absorption spectra of porcine and human corneas

Annamarie Lembares, Xin-Hua Hu, Gerhard W. Kalmus

Proceedings Volume 2971, Ophthalmic Technologies VII; (1997) https://doi.org/10.1117/12.275123
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We have measured the absorption spectra of porcine and human corneas in the far ultraviolet region from 350 nm to 190 nm. The samples were sectioned from frozen corneas into thin slices of 15 micrometers to 40 micrometers in thickness. The absorption spectra shows three distinct regions from 260 nm to 190 nm: a relatively weak absorption region from 260 nm to 240 nm; a region of steep increase from 240 nm to 220 nm; and a strong and slowly increasing absorption region from 220 nm to 190 nm. The linear absorption coefficients were determined to be 2130 +/- 310 (cm^-1) at 220 nm, 2240 +/- 320 (cm^-1) at 215 nm, 2300 +/- 330 (cm^-1) at 210 nm, and 2410 +/- 370 (cm^-1) at 193 nm for porcine corneas and 2300 +/- 480 (cm^-1) at 220 nm, 2300 +/- 460 (cm^-1) at 215 nm, 2320 +/- 470 (cm^-1) at 210 nm, and 2340 +/- 450 (cm^-1) at 193 nm for human corneas. A `window of ablation' in the far ultraviolet region between 220 and 190 nm was determined. Statistical analysis was conducted to correlate the far ultraviolet absorption between the porcine and human corneas. The effect of freezing on the far ultraviolet absorption also has been studied.

Citation Download Citation

Annamarie Lembares, Xin-Hua Hu, and Gerhard W. Kalmus "Far-ultraviolet absorption spectra of porcine and human corneas", Proc. SPIE 2971, Ophthalmic Technologies VII, (26 May 1997); https://doi.org/10.1117/12.275123

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