Hierarchical watershed transformation based on a-priori information for spot detection in 2D gel electrophoresis images

Susan Wegner; Klaus-Peter Pleissner; Helmut Oswald; Eckart Fleck

doi:10.1117/12.348632

21 May 1999 Hierarchical watershed transformation based on a-priori information for spot detection in 2D gel electrophoresis images

Susan Wegner, Klaus-Peter Pleissner, Helmut Oswald, Eckart Fleck

Author Affiliations +

Proceedings Volume 3661, Medical Imaging 1999: Image Processing; (1999) https://doi.org/10.1117/12.348632
Event: Medical Imaging '99, 1999, San Diego, CA, United States

Abstract

For the spot detection in 2D electrophoresis images an approach which is based on the combination of the watershed transformation (WST) with a-priori knowledge is presented. To identify spot regions in the over segmented result of the WST two types of regions have to be found: Regions that correspond to a complete spot and regions that cover only a part of a spot. The first localization step, the gray value analysis, is based on the assumptions that spot regions have significantly higher gray values than the background and that they border on a background region. Since not all remaining regions are spot or partial spot regions, additionally a curvature analysis is done. Here the a-priori knowledge is used that regarding a gel image as a surface, the shape of a spot is obviously convex. Consequently, considering the second derivative all required spot and partial spot regions can be obtained by the regions of convex curvature. In a final merging step all partial spot regions covering one spot have to be combined to only one spot region. As merging criterion two spot characteristics are used. A spot should have an approximately elliptical shape and partial spot regions of one spot should have a local convex curvature in a small neighborhood along their boundary.

Citation Download Citation

Susan Wegner, Klaus-Peter Pleissner, Helmut Oswald, and Eckart Fleck "Hierarchical watershed transformation based on a-priori information for spot detection in 2D gel electrophoresis images", Proc. SPIE 3661, Medical Imaging 1999: Image Processing, (21 May 1999); https://doi.org/10.1117/12.348632

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available